Coding

Part:BBa_K3978001:Design

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


GRIP 1, a protein which can bind to estrogen receptor（ER）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 276
    Illegal BglII site found at 2361
    Illegal BglII site found at 2406
    Illegal BglII site found at 2454
    Illegal BglII site found at 2637
    Illegal BglII site found at 2673
    Illegal BglII site found at 2709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2521


Design Notes

Insert the gene of GRIP 1 between the restrictions EcoRI and BamHI


Source

The sequence of GRIP 1 comes from PDB.

References